JC-1 has also been used in a functional assay of multidrug-resistance cells and in apoptosis-related mitochondrial modifications. Biochem/physiol Actions. Cell permeable: no. Primary Target Used as a ratiometric indicator of mitochondrial potential δΨm in cells, tissues, and isolated mitochondria.
Standard methods for JC-1 detection are not optimal. L1210 cells were first incubated with 1 μM valinomycin or DMSO (control) for 30 min at room temperature (RT) and then stained with 2.5 μM JC ...
JC-1 is widely used for determining mitochondrial membrane potential with flow cytometry. It is capable of selectively entering mitochondria, and reversibly changes its color from …
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In non-apoptotic cells, JC-1 accumulates as aggregates in the mitochondrial membranes, resulting in red fluorescence. The brightness of red fluorescence is proportional to the potential and varies among different cell types. However, in apoptotic and necrotic cells, which have diminished mitochondrial membrane potential, JC-1 exists in the ...
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Enhanced Signal: JC-10™ has a higher signal-to-background ratio than JC-1. Enhanced Sensitivity: JC-10™ has the ability to detect subtle changes in ΔΨm loss better than JC …
Cayman's JC-1 Mitochondrial Membrane Potential Flow Cytometry Assay Kit can be used to study mitochondrial behavior in a variety of conditions, including apoptosis. Changes …
JC-1 and JC-9 are cationic dyes (Figure 1) that exhibit potential-dependent accumulation in mitochondria, indicated by a fluorescence emission shift from green (~525 nm) to red …
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JC-1 is a membrane-permeant dye that selectively accumulates in the mitochondria and exhibits a shift in emission color from green to red as the mitochondria membrane potential becomes more polarized. Thermo …
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JC-1 fluorescence is usually excited by the 488 nm laser wavelength common in flow cytometers. In this study, we show that in practice this approach is not …
JC-1 is a membrane-permeable cationic dye that is used to study mitochondrial integrity in the context of cellular apoptosis. 1,2,3 It selectively enters mitochondria and changes fluorescence characteristics with alteration in mitochondrial transmembrane potential (ΔΨm).In healthy cells with a high mitochondrial ΔΨm, JC-1 forms complexes known as …
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Mitochondrial membrane potential determination using JC-1. Inoculate cells into a dish or a chamber slide and incubate the cells at 37 ℃ in a 5% CO 2 incubator.; Add an appropriate volume of JC-1 working solution to wells. *1 Incubate the cells at 37 ℃ in the 5% CO 2 incubator for 30–60 minutes.; Discard the supernatant and wash the cells with medium …
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Background. Apoptosis is a cellular process which involves genetical events causing the death of a cell. Several major events occur in mitochondria, of which, the most significant is the loss of mitochondrial transmembrane potential (ΔΨ M) (Green and Reed, 1998).Throughout the life of a cell, the mitochondria uses oxidizable substrates to …
JC-1 is a novel cationic carbocyanine dye that accumulates in mitochondria. The dye exists as a monomer at low concentrations and yields green fluorescence, similar to fluorescein. At higher concentrations, the dye forms J-aggregates that ex
A dual-emission potential-sensitive probe that can be used to measure mitochondrial membrane potential. JC-1 is a green-fluorescent (λ ex 520 nm) monomer at low membrane potential. At higher potentials, JC-1 forms red-fluorescent (λ em 596 nm ) "J-aggregates," which exhibit broad excitation and very narrow emission spectra. The ratio of red to …
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be tested starting around 2 μM JC-1. CCCP controls should be used to confirm that the JC-1 response is sensitive to changes in membrane potential. Reagent Preparation Allow the JC-1 powder and DMSO solutions to come to room temperature before use. Prepare a 200 μM JC-1 stock solution immediately prior to use by dissolving the contents of
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Mitochondrial membrane-potential dye. Exhibits potential-dependent accumulation in mitochondria. Fluorescence emission shift from green (~529 nm) to red (~590 nm). …
JC-1 is widely used for observing mitochondrial membrane potential, shows fluorescence characteristic change from green (530 nm) to red (590 nm) depending on the …
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Application Data: Simultaneously evaluation of mitochondrial superoxide and membrane potential . After HeLa cells were washed with HBSS, co-stained with MitoBright ROS Deep Red and mitochondrial membrane potential staining dye (JC-1: code MT09), and the generated mitochondrial ROS and membrane potential were observed …
